Mutagenesis can be induced by a variety of chemical agents, with a specific array of favored lesions and mutation frequencies. Chemical mutagenesis is a whole-genome profiling of mutagenesis for forward genetic screens and target-selected mutagenesis. This approach is the easiest and most straightforward way to be scaled up for systematically inducing germline mutations at high frequency, providing a new strategy for the breeding of new varieties and the establishment of animal models of diseases.
The genome sequencing of C. elegans has been achieved. Moreover, because of its hermaphroditism, severe mutations can be maintained homozygously in the population and hence their propagation through self-fertilization. Thus C. elegans is optimal for studying thoroughly the characteristics of each gene to get insights into the cellular processes. We offer chemical mutagenesis services in C. elegans.
We provide a rapid and scalable platform for high-throughput screening to identify mutants with two widely used chemical mutagenesis. The C. elegans growth, mutagenic procedure, screening, and mutant recovery are all in 96-well microtiter plates.
Ethyl methanesulfonate (EMS) is an alkylating agent, and its mutagenic properties were first demonstrated using the T4 viral system in 1959. With its relatively low toxicity and relatively good efficiency, EMS is the most commonly used chemical mutagen to mutate the genome of C. elegans and the most potent at the present.
The chemical N-ethyl-N-nitrosourea (ENU) is an alkylating agent, and has been used as a mutagen since the 1970s and for C. elegans mutagenesis in 1997. Compared to EMS, ENU has been shown to produce a different spectrum of mutations, especially for the production of non-null alleles, and it has greater toxicity to C. elegans. Its toxicity can be reduced in solvents such as absolute ethanol, and increased in aqueous solutions.
|Method||Types||Forward Mutation Rate||The mutation spectrum||Main Targets||Purposes|
|Chemical mutagenesis||EMS||as high as 2.5 × 10−3 mutations/gene/generation||Primarily G/C to A/T transitions||the sperm and oocytes||Forward genetic screens, target-selected mutagenesis|
|ENU||approximate rate of 1 per 700 gametes (Point Mutations Rate)||A/T to T/A transversions, G/C to A/T transitions, and A/T to G/C transitions||spermatogonial stem cells|
Creative Biogene strives for solutions that improve research outcomes and dramatically improve the speed of success. Based on the high-throughput genetic screes platform, we can assist you to induce and screen mutations of a specific phenotype in C. elegans with a rapid and scalable procedure. We are committed to offering the most affordable and fastest mutagenesis service in a timely and professional manner to ensure your satisfaction. If your research is in this area, please contact us for more details.
* For research use only.